Stem Cell

Biography

Robert Bowen graduated from the University of Illinois, School of Medicine and trained in internal medicine, critical care, and pulmonary medicine at the University of Virginia and West Virginia University (WVU) medical centers. He was trained in Laser Bronchoscopy in 1982/1983 and after completing a Fellowship in cosmetic anti-aging and regenerative medicine extended his laser experience to include aesthetic therapies. He is currently the Professor of Medicine at WVU and Director of the Center for Wound Care and Hyperbaric Medicine at WVU Berkeley Medical Center, USA. His current research interests include the translation of adipose derived stem cell treatments and light-based therapies into clinical applications in wound care and cosmetic medicine.

Abstract

Introduction: Lipoaspirate has shown great promise as a source of progenitor cells for use in regenerative medicine. The stromal vascular fraction (SVF) can be isolated from lipoaspirate using enzyme digestion and centrifugation, but this approach may be limited by the labour-intensive nature of the technique as well as ambiguities in current governmental regulations. An alternative approach to obtain SVF from lipoaspirate was studied.

Methodology: Paired (collected from contralateral regions) lipoaspirate specimens were acquired from 30 consenting patients (age 24-62; 22 females, 8 males) by suction-assisted liposuction (SAL) and nutational infrasonic liposuction (NIL). The infranatant from 50 ml of adipose tissue (LAF) was centrifuged at 400 g×5 min and the resultant pellet was collected with a pipette. Time=15-20 min. The respective SVFs cell populations were counted using an optical fluorescent cell counter (Nexcelom A2000) and the fluorescent stains-acridine orange (AO) and propidium iodide (PI).

Results: The number of nucleated, live cells from SAL infranatant was 97,345±23,435 per ml of adipose tissue and from NIL infranatant was 335,621±81,274 per ml of adipose tissue. The p value is <0.00001, n=30.

Conclusions: Regenerative cells can be isolated from the lipoaspirate infranatant from either SAL or NIL, although in lower quantities than from enzyme digestion. NIL acquisition yielded 3.5×the number of cells over that acquired from SAL. The time, skill, and cost of producing SVF from infranatant is less than using enzyme digestion, which potentially make these regenerative therapies accessible to more physicians and patients.

Biography

Malyshev Igor is a Head of the Department of Pathophysiology and Head of the Laboratory of Cell Biotechnology, Medical School at the Moscow State University of Medicine and Dentistry; 2. Head of the Laboratory of Stress, Institute of General Pathology and Pathophysiology, Moscow and 3. Adjunct Professor of Biomedical Sciences, University of North Texas Health Science Center, USA. He is a Member of the board of directors of the International Society for Adaptive Medicine and an Editorial board member of Journal of Biosciences and Medicines. He has published 3 books and monographs and 153 full length articles.

Abstract

Many tumors produce anti-inflammatory cytokines, which reprogram antitumor M1 macrophages to protumor M2 macrophages via activation of transcription factors, STAT3, STAT6, and SMAD3. Earlier we showed that М1 macrophages with inhibited STAT3, STAT6, and SMAD3 (M3 phenotype) responded to the action of protumor, anti-inflammatory cytokines by increasing production of antitumor, proinflammatory cytokines and thus, preserved their antitumor properties. In vivo, the tumor also disorders the antigen presentation by macrophages and prevents formation of antigen-specific Т and Th1 lymphocytes with strong antitumor properties. We hypothesized that presentation of tumor antigens to lymphocytes by M3 macrophages in vitro, in absence of tumor cells, could result in an effective antitumor programing of the lymphocytes. It can be expected that a composite pool of M3 macrophages and in vitro antigen-reprogramed lymphocytes would effectively restrict tumor growth. We showed that the antitumor effect of M3 macrophages depended on timing of their administration following the onset of solid tumor development. In early administration, M3 macrophages partially restricted the tumor development. In late administration, M3 macrophages restricted the tumor growth but to a significantly less extent. Adding antigen-reprogramed lymphocytes to M3 macrophages resulted in complete inhibition of tumor growth both in vitro and in vivo, both in early and late administration. The fact that M3 macrophages and antigen-reprogramed lymphocytes completely suppressed tumor growth makes it very promising to develop a clinical biotechnology for reducing the tumor growth by prior in vitro antitumor programing of the immune response.

Biography

Bruno Zelic received his PhD in chemical engineering in 2003 at University of Zagreb. From 2012 he is full professor at the Faculty of Chemical Engineering and Technology, University of Zagreb. His research interests are in the field of implementation of microreactor technology into biotechnology and biofuels production. More than 80 scientific and professional publications, 2 patents, and more than 60 oral and poster presentations on the international conferences present his scientific work. Bruno Zelić was dean of the Faculty of Chemical Engineering and Technology, University of Zagreb from 2013 to 2017. He is Editor-in-Chief of Chemical and Biochemical Engineering Quarterly journal.

Abstract

The need for the production of biofuels from various renewable sources is becoming increasingly interesting especially as the availability and accessibility of fossil fuels is significantly declining. Biodegradability, low pollution emissions and non-toxicity of raw materials are some properties making biogas, biodiesel and bioethanol more environmentally friendly fuels. Solid-state fermentation could be a suitable technology for the production of value-added products by utilization of the renewable waste materials, which makes it also economically feasible. So far, this technology was used for production of enzymes, organic acids, mushrooms, flavour and aroma compounds, pigments, polysaccharides, hormones, human food and animal feed. Different type of bioreactors have been developed and successfully used for solid-state fermentation of broad range of substrates and in production of value-added products. Solid-state fermentation will be demonstrated as part of anaerobic degradation on lab-, pilot- and industrial-scale of several waste materials such as brewer's spent grain, whey and cow manure, and corn silage and cow manure, respectively. The application of different microreactor systems in intensification of the biodiesel production process is widely studied. However, previous studies of the application of microreactor technology in the production of biodiesel were limited to the use of chemical catalysts. Mild reaction conditions, absence of by-products, reusability, simple separation and purification of the resulting biodiesel as well as lower energy consumption are some of the many advantages that make the enzyme lipase – a biocatalyst – a better choice than traditional chemical catalysts in the process of biodiesel production. Different microreactor systems utilising a commercially available lipase and a lipase produced by solid-state fermentation were used for transesterification of fresh and waste cooking oil while biodiesel was separated using integrated microseparation unit. Selected examples are clear demonstration of environmentally friendly and economic technologies used for efficient production of biofuels on micro-, lab-, pilot- and industrial scale.

Biography

Asst. Prof. Oliver Spadiut studied Food Science and Biotechnology at the University of Natural Resources and Life Sciences (Vienna). After 18 months of successful post-doctoral research at the Royal University of Technology KTH in Stockholm, Sweden, he became University Assistant at TU Wien, Austria. Dr. Spadiut received his Habilitation in Biotechnology in March 2015 and is currently Asst. Prof. at TU Wien and group leader of the research group "Integrated Bioprocess Development".

Abstract

Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive aggregates of misfolded protein, and thus should be avoided during recombinant protein production, numerous bio pharmaceutically important proteins are currently produced as IBs. To obtain correctly folded, soluble product, IBs have to be processed, namely harvested, solubilized and refolded. Several years ago, it was discovered that, depending on cultivation conditions and protein properties, IBs contain partially correctly folded protein structures, which makes IB processing more efficient. I present a method of tailored induction of recombinant protein production in E. coli by a mixed feed system using glucose and lactose and its impact on IB formation. My method allows tuning of IB amount, IB size, size distribution and purity, which does not only facilitate IB processing, but is also crucial for potential direct applications of IBs as nanomaterials and biomaterials in regenerative medicine.

Biography

Mr Haamid Bashir is pursuing Doctorate (PhD) in Biological Science from University of Kashmir, Hazratbal Srinagar through Research centre Department of Biochemistry, Government Medical College Srinagar. Haamid has published more than 15PubMed indexed publications in reputed national and international journals. One of publication is published in European cancer and prevention journal (impact factor 3.5). Haamid has expertise in molecular genetics and epigenetics in cancer biology, Thyroid, Diabetes, Metabolic syndrome. Haamid has attended many national conferences and workshops. Haamid has been associated with number of scientific journals.

Abstract

Background: Subclinical hypothyroidism is defined as elevated serum TSH levels with normal levels of T3 and T4 hormones. Vitamin D is an important vitamin that not only regulates calcium, but also has many other beneficial actions like immune modulator. The aim of study is to find the relationship between Subclinical hypothyroidism and vitamin D levels in ethnic population of Kashmir, India.

Subjects and Methods: Thyroid hormones (FT3, FT4, TSH, T3, T4, Anti thyroid peroxidases) and vitamin D (25-OH) levels were measured in 80 patients with Subclinical hypothyroidism and 70 healthy subjects, by Chemiluminisence method on Abbott USA I 1000SR Automatic immunoassay analyzer in Department of Biochemistry, Govt Medical College, Srinagar.. Vitamin D deficiency was designated at levels lower than 30 ng/ml. Calcium levels were also evaluated in all participants on biochemistry analyzer Abbott USA c4000.

Results:  Serum 25(OH) vit D was significantly lower in Subclinical hypothyroid patients than in healthy controls (F=27.7, P<0.0001). Its level was significantly decreased in females than male patients (t=1.2, P<0.05).Calcium levels was also a significant decrease in subclinical hypothyroid patients when compared to controls (t=1.5, P<0.05).

Conclusion: On the basis of present study in mountainous valley of Kashmir subclinical hypothyroid patients are suffered from hypovitaminosis D and hypocalcaemia that is significantly associated with the degree and severity of the Subclinical hypothyroidism. Thus we suggest that patients are to be encouraged for vit D supplementation and we recommend that the mass screening for Vitamin D, Thyroid hormone profile and serum calcium levels in this ethnic population of Kashmir.

Biography

Dr. Tamar Varazi‘s qualification - chemist -  complated at Tbilisi State University of Georgia. She received her scientific degree – PhD in Biochemistry at the Institute of Biochemistry and Biotechnology of Georgia.Current ocupation is Senior scientist and Full Professor of  Georgian Agricultural University, Durmishidze Institute of Biochemistry and Biotechnology, Department of Biological Oxidation. She has scientific experiences in Investigation of biochemical principles of phytoremediation of polluted environment. She is a manager, coordinator and researcher of many scientific and applied international projects. She has highly developed presentation/teaching skills. Her publications includes 3 Monographs and  47 articles in international and local scientific journals.

Abstract

The complex technological approach to the fast removal of ecotoxicants from ecosystems is a very urgent matter for all countries. The development of water remediation methods for the removal of chemical contaminants is a challenging problem. One of the methods to clean chemically polluted waters is using of algae (the so-called Phycoremediation). Spirulina (Spirulina platensis) should have prospects for phytoremediation of waters polluted by different toxic compounds. Evaluation of ecological potential of Spirulina, in particular, its tolerance and detoxication ability towards organic ecotoxicants and heavy metals, is the novelty in sphere of researches in xenobiochemistry.

Recently intensive study of contaminated areas has revealed special property of bacteria living in these areas. The processes of microorganism’s biostabilization of soluble forms of hazardous pollutants can be used for bioremediation of soils, contaminated by oil, Pesticides, heavy metals and radionuclides. Monitoring of bacterial consortiums in contaminated regions will enable to assess in advance the extent of native bioremediation and accordingly suggest a strategy of detoxification. For this purpose we have developed the phylogenetic oligonucleotide low-density biochip based on the 16SrRNA genes sequences.

The goal of presented work is to develop of quick response strategy and effective flexible technology of targeted toxicants removal from polluted water and soils. The approach is based on joint action of microorganism and plants with high detoxification potential, using natural minerals with function of a sorbent is to uptake and to trap pollutants emission in the environment and biochips – tool for bioaugmentation of different type of toxicants.

Biography

Zeliha Selamoglu is a Professor in Medical Biology department of Nigde Ömer Halisdemir University, Turkey. She earned her PhD in Biology from Inonu University, She has published over 90 peerreviewed journal articles with over 760 citations and many technical reports. She is a member of Society for Experimental Biology and Medicine: Associate Membership and European association for cancer research. She has served as Editorial Board member for many Journals.

Abstract

To investigate changes in adult female rat ovary tissue following acrylamide (AA) and vitamin E administration during pregnancy. The present study was conducted with the approval of the experimental animals ethics committee at Inonu University, Faculty of Medicine (2017 / A-11). Thirty rats, confirmed to be pregnant with vaginal smear, were divided into 5 different groups that included equal number of pregnant rats: Control, Corn oil, Vitamin E, Acrylamide, Vitamin E + Acrylamide Groups. The birth was monitored on the 21st day of gestation and female rats were selected and at the end of 8 weeks, the rats were decapitated. Rat ovary tissues were examined for malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant status (TAS), total oxidant status (TOS) and superoxide dismutase (SOD), catalase (CAT) and nitric oxide (NO) levels. It was determined that AA had a negative effect on oxidant-antioxidant parameters (MDA, GSH, NO, SOD, CAT, TAS, TOS) in the ovary tissue (P<0.05), whereas Vitamin E administration increased GSH, TAS, NO, SOD, CAT levels (P<0.05). It is unlikely to be exposed to food-borne AA toxicity and AA toxicity could lead to permanent damages. It is certain that infertility incidences are increasing among the population every day. Vitamin E is observed to have protective effects against AA toxicity, however further studies are required.

Biography

Mija Sezun has completed her PhD on biological and biotechnological sciences. Her doctoral thesis included the environmental biotechnology area. At the moment she is working at the Pulp and Paper Institute and mainly deals with biotechnology in the paper industry through the use of enzymes in the process of paper production. Currently researching the production of enzymes by using fungi by applying paper mill sludge, as the substrate for the cultivation of fungi. In addition to the fungal enzyme production she also deals with the use of commercial enzymes to improve the efficiency of processes in the paper industry.

Abstract

The largest by-product in pulp and paper industry is sludge and disposal is major solid waste problem for the industry (Battaglia et al., 2003; Geng et al., 2006). It was predicted that global production of paper mill sludge rise over the 50 years by between 48 and 86% over current levels (Mabee & Roy, 2003). The landfilling of waste paper mill sludge has become less achievable in recent years as environmental concerns have lead to rapidly increasing costs. Sanchez (2010) report that the mushrooms have economical, ecological and medicinal values. Their advantage is also that they are able to colonize and degrade a large variety of lignocellulosic materials and other wastes which are produced in agricultural, forest and food-processing industries. We studied the growth of oyster mushroom (Pleurotus ostreatus) on pulp and paper industry solid wastes. During the study, we investigated whether solid-state fermentation and growth substrates (de-inking paper mill sludge, primary sludge) are appropriate for P. ostreatus production of enzymes relevant to the pulp and paper industry. Following fermentation, extracellular protein was extracted and the specific activities of four enzymes were determined: the cellulase, xylanase, lipase, and peroxidase activities. These enzymes are used in the paper industry for the improvement of various industrial processes and in many other applications. Results show that P. ostreatus can grow on pulp and paper industry solid wastes, which will help to minimize the waste volume, and to decrease the ecological impact. Furthermore, these pulp and paper industry solid wastes are good substrates for the production of commercially interesting enzymes. 

Biography

Mojgan Ahmadzadeh Raji has completed her PhD from University of Tehran as a common project with York University, Toronto, Canada with my supervisor prof. Ebrahim Ghafarzadeh from Department of Electronic and Computer Science in York University .She is a Nano researcher in Faculty of Veterinary Medicine, University of Tehran. He has published more than 23 papers in reputed journals and has been serving as an Expert Reviewer for IEEE Conference and Prostate Cancer Foundation of Australia.

Abstract

The English surgeon Stephen Paget has a seed and soil hypothesis for the description of metastases which Circulating Tumor Cells (CTCs) are considered as seeds when they can spread in the body to find a place for growth. Adeline Sadeli belief that “the challenge to detect CTCs in peripheral blood of cancer patients is as difficult as finding needles in a haystack”, finding a low cost, specific and accurate method for CTCs detection and separation; is regarded as a significant challenge for many researchers in Nano biotechnology and electrical engineering. Design and implementation of Nano biosensors with the aim of CTC detection can be useful to monitor the effective treatment for cancers and help to choose the best treatment method. Technological advances offer the solutions to facilitate the detection of rare cells. Cell search (Veridex) is a detection system in whole blood, which is based on magneto beads coated with antibody against cytokeratin. In this regard, aptamers are considered as competitors for antibodies.  They possess a series of properties, including stable structure, no immunogenic and nontoxic structure, easily chemical modification, facile synthesis without using animals, leading to reducing the cost of production. Hence, aptamers have been used for molecular targeting applications.  With this respect, due to appropriate electrical conductivity and electron transfer, gold nanoparticles are used as a foundation for aptamer detection with linkers and thiol groups.  Indium tin oxide with transparent property and gold nanoparticle immobilization ability through electrodeposition is a well candidate for cell separation and provide an opportunity for microscopic cell visualization. The objective of this research work is obtain a transparent support with gold nanoparticles immobilized on Indium tin oxide for aptamer attachment to separate HCT 116 colon cancer cell line.

Biography

Elodie Czuba is a PhD student in European Diabetes study center. She is interested in nanoencapsulation of drugs for a biological application

Abstract

Introduction: Poly (D, lactic-co-glycolic) acid (PLGA) nanoparticles (NP) are known to be effective drug carrier with a long acting profile but have less than 20% of bioavailability.

Aim: The aim of this work was to increase encapsulated insulin bioavailability modifying the surface properties like charge of NPs to improve their interaction with cell membrane.

Materials & Methodology: Particles were prepared in presence or not of polyvinyl alcohol (PVA), with Sodium dodecyl sulfate (SDS) or coated with chitosan chloride to play on NPs charge. In vitro internalization was tested on epithelial coculture (Caco-2/RevHT29MTX) by flow cytometry. NPs were then administrated thanks to a pharmaceutical complex vector in vivo by oral route (10, 20 and 50 UI) in diabetic rats.

Results: SDS-NPs and 1% PVA-NPs were smaller (141±3 and 154±24 nm) than chitosan coated NPs with size (236±29 nm) which is increased in comparison to the control (200±9 nm). Compared to classical NPs (PVA+), cells uptake was improved by SDS-NPs, in contrast to without PVA and chitosan coated NPs. Administration by oral route of vehicle contained SDS-NPs 20 and 50 UI) reduced glycaemia faster than empty vehicles and vehicles with standard NPs. Negative charge of NPs could interact with cell membrane which is negatively charged too.

Conclusions: Mucoadhesive particles (chitosan) formulation is definitely not the good approach to improve the bioavailability of encapsulated insulin contrary to negative NPs are the most efficient both in vitro and in vivo and represent a promising formulation for oral insulin delivery.

Biography

Magdalena Arévalo Turrubiarte has a bachelor in veterinary medicine, she obtained her MSc in Animal Production and Health at the Universidad Nacional Autonoma de México (UNAM) focusing on adipogenesis in beef cattle. She completed her PhD at the Agrocampus Ouest-INRA, France with specialization in biology and agronomy working epithelial cell phenotype in the mammary gland bovine during a lactation period. Currently, she is working with the group of Veterinary Physiology at University of Turin in Italy in a project with the use of stem cells in tissue regeneration in equines. She has done farm practice and some laboratories internships in Mexico and in the United States of America.

Abstract

Mesenchymal cells (MSCs) have been used in regenerative medicine as an option to restore damaged tissues mostly in locomotory equine problems. MSCs can be obtained from different tissues in equines. Previous studies have shown that source sites remain decisive for MSCs yield, cell viability and might influence cell behaviour in vitro and in vivo. To our knowledge, other studies have used cells from bone marrow, adipose tissue, and synovial fluid. However, an in-depth study from this different type of sources and a comparison among them in respect to MSCs in vitro performance and phenotype is lacking. The aim of this study was to evaluate through flow cytometry cell phenotype by cell surface markers (CD11α/CD18, CD45, CD79α, CD90, CD105 and MHC II) reported in equine MSCs. We completed this study with in vitro functional differentiation assays to confirm their mesenchymal identity. The evaluation of  cell in vitro performance by different test (cell growth and migration) was made. We observed differences in tissue sources such as cells isolated from synovial fluid had a higher growth rate than bone marrow and adipose cells. Cell populations showed differences in the expression of surface markers, thus giving information about specific cell phenotypes. Adipose tissues had a highest expression of CD105 than bone marrow and synovial fluid cells. Adipose and bone marrow seem to be the main tissues used to obtain MSCs, however, synovial fluid cells have a closer chondrocytelike profile which might be a better option to specific joint issues. Among the different type of adipose cells, neck fat tissue seems to be a more feasible choice to obtain MSCs in addition to the amount of cells and cell performance characteristics. This study should help in the selection of a more appropriate tissue harvesting source in the use of MSCs for cell therapy in equines.

Biography

P Setthawong has completed her Bachelor's Degree in Faculty of Veterinary Science (First-Class Honors) from Chulalongkorn University, Thailand. She got a Scholarship in Thailand Research Fund through the Royal Golden Jubilee PhD Program (Grant No. PHD/0143/2556). She is currently pursuing a Doctor of Philosophy Degree in the field of Obstetrics, Gynaecology and Reproduction at Chulalongkorn University, Thailand. She is interested in veterinary stem cells especially in pig as an animal model for human. She aims to study about generation of embryonic stem cells and induced pluripotent stem cells. She has succeeded in establishing and characterizing induced pluripotent stem cells derived from Sertoli and fibroblast cells.

Abstract

Induced pluripotent stem cells (iPSCs) are generated by reprogramming of fully differentiated adult cells using four transcription factors, including OCT4, SOX2, KLF-4 and c-MYC (OSKM). Porcine is a meaningful model for regenerative medicine because its anatomy and physiology are similar to human. However, reprogramming efficiency of porcine fibroblasts into iPSCs is currently poor. This study used Sertoli cells as a novel cell origin for somatic cells reprogramming. Neonatal testes were collected from 1-week old piglets. The testes were digested by two-step enzymatic method in order to isolate the Sertoli cells. The Sertoli cells were transfected with retroviral vectors expressing OSKM. We observed the primary colonies and counted on day 7 after transfection. The characteristics of Sertoli iPSCs-like colonies were analyzed by morphology, alkaline phosphatase staining, RT-PCR, G-banding, in vitro and in vivo differentiation. Sertoli cells obtained from neonatal porcine showed typically polygonal shaped. A total of 240 colonies (0.33%) originated from seeding 72,500 cells were observed on day 7. The Sertoli iPSCs-like colonies exhibited a high nuclear per cytoplasm ratio with prominent nucleoli. We picked up 30 Sertoli iPSCs-like colonies and 8 cell lines (26.6%) demonstrated undifferentiated stage of iPSCs. The Sertoli iPSCs-like colonies were positive to alkaline phosphatase staining and expressed endogenous pluripotent. G-banding analysis demonstrated normal karyotype. Under differentiation conditions, iPS-like cell lines could form three-dimension aggregated masses, which represented three germ layers of embryonic cells. For in vivo differentiation, tumour mass were collected and presented all of ectoderm, mesoderm, and endoderm. In conclusion, the Sertoli cells can be used as a novel somatic cell origin for iPSCs reprogramming.

Biography

Saadia Ijaz is a Young Researcher. She has just completed her PhD in April, 2017 from The University of the Punjab, Lahore Pakistan. Currently she is working as Assistant Professor in The Women University, Multan Pakistan. Her main research interest is in the role of Cyanobacteria in biotechnology including but not limited to nutraceutical, pharmaceutical, biofuel production, environmental biosensors, cosmetics, biomedical research applications and harmful aspects of cyanobacteria. She has published 2 research papers in impact factor journals and also won Rs. 0.5 million Start-up research grant from Higher Education Commission of Pakistan for her work with anticancer carotenoids from Cyanobacteria.

Abstract

The 70% methanolic extract of Cyanobacterial strain; Oscillatoria sp. SI-SA containing phenolic compounds; Tannic acid, Orcinol, Pholoroglucinol, Salicylic acid, Acetyl Salicylic acid and Protocatechuic acid, was evaluated for its non-cytotoxic, non-genotoxic and non-mutagenic activities along with its anticancer potential against two (MCF-7 and MDA-MB-231) breast cancer cell-lines in order to study their chemotherapeutic effects without causing damage to normal cells. The strain was isolated from Kallar Kahar Salt Lake, Pakistan. It was identified by polyphasic approach including both morphological and molecular methodologies. The extract showed very low cytotoxicity against normal lymphocytes only at highest concentration of 1000 µg/ml with high IC50value of 1088 µg/ml. Comet assay also showed low genotoxicity only at highest non-cytotoxic concentration of 1000 µg/ml with 15.37% DNA damage. Ames fluctuation Salmonella typhimurium test against four mutant strains TA100, TA98, TA97a and TA102 also showed significant non-mutagenic effects of the extract with and without metabolic activation. Finally, the extract was evaluated for its anticancer potential which showed significant anticancer activities in dose-dependent manner against MCF-7 and MDA-MB-231 cell-lines giving low IC50 values of 61.75 and 82.75 µg/ml respectively. It was further observed that after three days of treatment with the extract, 19.29 and 26.81 % cell viability remained of MCF-7 and MDA-MB-231 cell-lines respectively at highest concentration of 250 µg/ml. The phenolic extract of Oscillatoria sp. SI-SA indeed showed promising anticancer activities without causing severe damage to normal cells and thus could be used as an alternative bioresource for anticancer therapeutics against breast cancers.