Stem Cell

Biography

Mojgan Ahmadzadeh Raji has completed her PhD from University of Tehran as a common project with York University, Toronto, Canada with my supervisor prof. Ebrahim Ghafarzadeh from Department of Electronic and Computer Science in York University .She is a Nano researcher in Faculty of Veterinary Medicine, University of Tehran. He has published more than 23 papers in reputed journals and has been serving as an Expert Reviewer for IEEE Conference and Prostate Cancer Foundation of Australia.

Abstract

The English surgeon Stephen Paget has a seed and soil hypothesis for the description of metastases which Circulating Tumor Cells (CTCs) are considered as seeds when they can spread in the body to find a place for growth. Adeline Sadeli belief that “the challenge to detect CTCs in peripheral blood of cancer patients is as difficult as finding needles in a haystack”, finding a low cost, specific and accurate method for CTCs detection and separation; is regarded as a significant challenge for many researchers in Nano biotechnology and electrical engineering. Design and implementation of Nano biosensors with the aim of CTC detection can be useful to monitor the effective treatment for cancers and help to choose the best treatment method. Technological advances offer the solutions to facilitate the detection of rare cells. Cell search (Veridex) is a detection system in whole blood, which is based on magneto beads coated with antibody against cytokeratin. In this regard, aptamers are considered as competitors for antibodies.  They possess a series of properties, including stable structure, no immunogenic and nontoxic structure, easily chemical modification, facile synthesis without using animals, leading to reducing the cost of production. Hence, aptamers have been used for molecular targeting applications.  With this respect, due to appropriate electrical conductivity and electron transfer, gold nanoparticles are used as a foundation for aptamer detection with linkers and thiol groups.  Indium tin oxide with transparent property and gold nanoparticle immobilization ability through electrodeposition is a well candidate for cell separation and provide an opportunity for microscopic cell visualization. The objective of this research work is obtain a transparent support with gold nanoparticles immobilized on Indium tin oxide for aptamer attachment to separate HCT 116 colon cancer cell line.