Stem Cell

Biography

Omid Koohi-Hosseinabadi has an associate degree in vet and also 2 bachelor degrees in biology and genetic counselling. He is doing master of Biochemistry in Islamic Azad University of Shiraz at the age of 36 years. He started to work at Laboratory Animal Center (recently known as Center of Comparative and Experimental Medicine), Shiraz University of Medical Sciences, Shiraz, Iran for 8 years, and also as a research assistant in Stem Cells Technology Research Center at Shiraz university of Medical Sciences since December, 2011 till December, 2017. At the moment he works at Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran. He has published more than 70 papers. Also has been serving as an editorial member of Journal of Fasa University of Medical Sciences. Omid is a particularly bright individual with good knowledge and the interpersonal skills required to relate that knowledge to others.

Abstract

Background: Osteoarthritis (OA) is a degenerative, progressive and painful synovial joint disease, which particularly occurs in the knee joint. The object of this essay is to evaluate the effect of Pistacia atlantica hydroalcoholic extract with rat bone marrow stem cells in induced osteoarthritis rat model. Methodology: Fifty adult male Sprague - Dawley rats after OA induction were divided into five groups of 10 rats as: control group without OA, OA + injection of bone marrow mesenchymal stem cells (BMSCs), OA + injection of hyalgan, OA + injection of Pistacia atlantica fruit extract, OA + injection of BMSCs with Pistacia atlantica fruit extract. Then, by histopathological, radiography and biochemical examination were evaluated. A P value <0.05 was considered as statistically significant. Results: In animals were administered BMSCs + Pistacia atlantica fruit extract, subchondral bone, cell distribution and articular cartilage surface were significantly increased compared with hyalgan and control groups (p<0.05). In BMSCs + Pistacia atlantica fruit extract group, osteophytes index of medial tibial condyle and medial fabella condyle, and total osteophytes were significantly less than hyalgan and control groups, while findings of osteophytes index about medial femoral condyle showed significant increased compared with control group (p<0.05). Serum TAC (total antioxidant capacity) and MDA (malondialdehyde) were significantly different in BMSCs, hyalgan and Pistacia atlantica fruit extract groups when compared separately with control group and all treatment groups (p<0.05). Conclusion: It seems that intra-articular synchronous injection of BMSCs and Pistacia atlantica fruit extract has an important role and remarkable function in repair and improvement of articular cartilage and also reduction and increment of MDA and TAC serum levels, respectively.